Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose

Jose Carlos Paredes Franco , Maria Lucia Sampaio Güther , Michael A J Ferguson

Glycobiology, Volume 35, Issue 2, February 2025, cwae097

Abstract

For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[³H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[³H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.