Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Sateriale A, Pawlowic M, Vinayak S, Brooks C, Striepen B

In: Mead JR, Arrowood MJ, editors. Cryptosporidium: Methods and Protocols [Internet]. New York, NY: Springer New York; 2020. p. 219–28


Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.